Analysis Note

ControlHeLa cell lysate

Application

Use Anti-Cyclin T1 Antibody (Sheep Polyclonal Antibody) validated in WB to detect Cyclin T1 also known as CDK9-associated C-type protein, cyclin C-related protein.

Western Blot Analysis: A 1:1000 dilution from a previous lot detected Cyclin T1 in 10 µg of HEK293, HeLa, HepG2, Jurkat, and PC3 cell lysates.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cyclin T1, which is also known as CCNT, is a member of the cyclin family and functions as a CDK kinase regulator. Cyclin T1 is one of regulatory cyclins (T1, T2a, T2b, and K) that is closely associated with CDK9 kinase forming a heterodimer called P-TEFb. This kinase complex is involved in phosporylating the carboxyl-terminal domain (CTD) of the large RNA polymerase II subunit which stimulate the elongation upon initiation. The cyclin T1/CDK9 complex has also been referred to as tat-associated kinase. Tat is an HIV protein that targets the cyclin T1/CDK9 complex to activate the transcription of the virus.

Immunogen

His-tagged recombinant protein corresponding to human Cyclin T1.

Epitope: Unknown

Physical form

Sheep polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected Cyclin T1 in 10 µg of HeLa cell lysate.

Specificity

Rat (85% sequence homology).

This antibody recognizes Cyclin T1.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Cyclin T1 is expected to be ~85 kDa This antibody detects a ~85 kDa band in most cell lines. It also detects the degraded forms of Cyclin T1 (~70 kDa, ~60 kDa, et al), which is beneficial for IP applications. According to independent laboratory, this antibody can immunoprecipitate multiple forms of Cyclin T1.