Analysis Note

ControlHuman lung tissue lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blot Analysis: 1 ug/ml of this antibody detected Ipaf in 10 ug of human lung tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ipaf in NIH/3T3 cells.

This Anti-Ipaf (NLRC4) Antibody is validated for use in IC, WB for the detection of Ipaf (NLRC4).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ipaf, also known as NLRC4, is a human CED-4 homologue and is thought to be an activator of pro-caspase-1 and might also be associated with the activation of caspase-1 as a response to pro-inflammatory and apoptotic stimulus. It has been shown to be induced by p53 and contributes to p53-dependent apoptosis. Ipaf is thought to play a role in the activation of the innate immune system.

Immunogen

Epitope: LRR4 domain

KLH-conjugated linear peptide corresponding to human Ipaf (NLRC4) at the LRR4 domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Ipaf in 10 µg of human lung tissue lysate.

Specificity

This antibody recognizes Ipaf at the LRR4 domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~112 kDa observed.