Analysis Note

ControlHuman skeletal muscle, fetal tissue lysate

Application

Anti-Protein patched homolog 1 Antibody detects level of Protein patched homolog 1 & has been published & validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Protein patched homolog 1 in kidney tissue.

Research CategorySignaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein patched homolog 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo).

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human skeletal muscle, fetal tissue lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Protein patched homolog 1 on 10 µg of human skeletal muscle, fetal tissue lysate.

Specificity

This antibody recognizes the cytoplasmic domain of Protein patched homolog 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 160 kDa