Analysis Note

ControlHeLa cell lysate

Application

Western Blot (SNAP i.d.™ Protein Detection System) Analysis: 5 µg/mL from a previous lot detected Interleukin-13 receptor, alpha-2 in 10 µg of HeLa cell lysate.

Immunohistochemistry (paraffin) Analysis: A 1:500 dilution from a previous lot was used to detect Interleukin-13 receptor, alpha-2 in colorectal carcinoma and normal human kidney/medulla tissue sections.

Research Sub CategoryCytokines & Cytokine Receptors

Anti-IL-13 receptor Antibody, alpha-2 (IL-13RA2) is an antibody against IL-13 receptor for use in WB, IH(P).

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-13 receptor subunit alpha-2 (IL-13RA2) is one receptor of Interleukin-13 which is a key moderator of asthma and allergic inflammation. IL-13 and its receptors have been shown to play a part in alcohol-mediated activation of pro-fibrotic pathways. IL-13 has a suppressive effect on macrophage activation but cannot activate T cells.

Immunogen

KLH-conjugated linear peptide corresponding to human Interleukin-13 receptor, alpha-2 at the cytoplasmic domain.

Epitope: Cytoplasmic Domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Interleukin-13 receptor, alpha-2 in 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes Interleukin-13 receptor, alpha-2 at the cytoplasmic domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~44 kDa observed