Analysis Note

ControlMouse brain microsomal fraction tissue lysate

Application

Western Blot Analysis: A previous lot detected Frizzled-7 on 10 µg of HEK293 cell lysate and rat brain microsomal fraction tissue lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-7 in human ductal in situ and GI tract tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Frizzled-7 in A431 cells.

Research CategorySignaling

This Anti-Frizzled-7 Antibody is validated for use in WB, IH, IC for the detection of Frizzled-7.

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Frizzled 7 is a multi-pass membrane protein belonging to the G-protein coupled receptor Fz/Smo family. It is a receptor for Wnt signaling, predominantly Wnt11, a crucial factor in the regulation of cellular migration and polarization during vertebrate gastrulation. Highest reported expression is in fetal kidney tissue and adult heart tissue. Lower levels have been observed in brain, placenta, adult heart, fetal lung, and squamous cell esophageal carcinomas.

Immunogen

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human Frizzled-7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse brain microsomal fraction tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Frizzled-7 on 10 µg of mouse brain microsomal fraction tissue lysate.

Specificity

Recognizes Frizzled-7

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

The calculated molecular weight is 64 kDa Observed at ~83 kDa, most likely due to multiple glycosolation sites.