Analysis Note

ControlUntreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for12 hours.

Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Anti-RIG-I, C-terminus Antibody is an antibody against RIG-I, C-terminus for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Retinoic acid-inducible gene 1 (RIG-1, DDX58) is a cytoplasmic protein belonging to the family of RIG-1-like receptor (RLR) family. It is a multidomain protein containing an N-terminal caspase recruitment domain (CARD); a DExD/H box helicase domain; and a C-terminal repression domain. RIG-1 has a well-documented role in innate immunity, as a virus-sensing protein that detects viral RNA molecules and induces the production of IFN-β through activation of the inflammasome complex.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human RIG-I.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for 12 hours.

Western Blot Analysis: 1 µg/mL of this antibody detected RIG-I in 10 µg of untreated A549 cell lysate and A549 cell lysate infected with 40 HAU/mL of SeV for12 hours..

Specificity

This antibody recognizes the C-terminus of RIG-I.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~102 kDa observed