Analysis Note

ControlHuman testis tissue lysate

Application

Research Sub CategoryHormones & Receptors

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Probable ergosterol biosynthetic protein 28 in HeLa cells.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in rat liver cells. (Malik, P., et al. (2010). Cell. Mol. Life Sci. DOI 10.1007/s00018-010-0257-2.)

This Anti-Probable ergosterol biosynthetic protein 28 Antibody is validated for use in WB, IC for the detection of Probable ergosterol biosynthetic protein 28.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Probable ergosterol biosynthetic protein 28, also known as NET51, is a very basic polypeptide containing several, evolutionary conserved transmembrane domains that are likely to play a role in sterol biosynthesis. It is found predominantly in normal testis and has also been found in many cancer cell lines and tumors.

Immunogen

KLH-conjugated linear peptide corresponding to human Probable ergosterol biosynthetic protein 28 near the first transmembrane domain.

Epitope: Transmembrane domain

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 1:220 dilution of this antibody detected Probable ergosterol biosynthetic protein 28 on 10 µg of human testis tissue lysate.

Specificity

This antibody recognizes Probable ergosterol biosynthetic protein 28 near the first transmembrane domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~16 kDa observed