Analysis Note

ControlHuman heart tissue lysate

Application

Western Blot Analysis: 1 µg/mL from a representative lot detected NET5 on 10 µg of rat heart tissue lysate.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory that detected NET5 in U2OS cells expressing various NET-GFP fusions. (Wilkie, G., et al. (2011) Mol. Cell Proteomics).

Research CategoryEpigenetics & Nuclear Function

Use Anti-NET5 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect NET5 also known as transmembrane protein 201.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transmembrane protein 201, also known as NET5, is a transmembrane protein of the nuclear envelope that is homologous to spindle-associated membrane protein 1 (SAMP1) in humans. NET5 is thought to be expressed on the inner nuclear membrane during interphase and move to the polar regions of the mitotic spindle during mitosis.

Immunogen

KLH-conjugated linear peptide corresponding to human NET5.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity purified

Quality

Evaluated by Western Blot in human heart tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected NET5 on 10 µg of human heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~72 and/or 43 kDa observed depending on lysate used. Isoform1: 72 kDa and Isoform 2: 43 kDa Uncharacterized band at ~28 kDa may be observed in some lysates.