Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated active Erk1 from lysate of NGF-stimulated (50 ng/mL) PC-12 rat pheochromocytoma cells.

Western Blotting Analysis: A representative lot detected Erk1 in human MCF-7 is a breast cancer cell lysate (Chen, J.Q., et al. (2013). Anal. Biochem. 442(1):97-103).

Western Blotting Analysis: A representative lot detected Erk1 in human HaCat keratinocyte lysate (Jarosz, M., et al. (2012). PLoS One. 7(6):e39436).

Western Blotting Analysis: A representative lot detected Erk1 in HT-29 human colorectal adenocarcinoma cell lysate (O′Neill, R.A., et al. (2006). Proc. Natl. Acad. Sci. U. S. A. 103(44):16153-16158).

Research CategoryEpigenetics & Nuclear Function

This rabbit monoclonal Anti-ERK1/MAPK3 Antibody, CT, clone UP-158-22-3-10, Cat. No. 05-957-I detects levels of ERK1/MAPK3, and has been published and validated for use in Immunoprecipitation and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mitogen-activated protein kinase 3 (EC 2.7.11.24; UniProt P21708; also known as ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, MNK1, p44-ERK1, p44-MAPK) is encoded by the Mapk3 (also known as Erk1, Prkm3) gene (Gene ID 50689) in rat species. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and -Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat ERK-1 (MAPK 3).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Unpurified.

Rabbit monoclonal antibody IgG hybridoma culture supernatant without preservatives.

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this hybridoma culture supernatant detected Erk1 in 10 µg of mouse NIH/3T3 cell lysate.

Specificity

Clone UP-158-22-3-10 recognizes a C-terminal epitope of MAPK 3 (ERK1). Immunogen sequence is not present in human MAPK3 spliced isoform 3 (ERK1b).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~44 kDa observed. 43.00/38.14 kDa (human MAPK3 isoform 1/2), 42.95/45.64 kDa (rat MAPK3 isoform 1/2), 42.93 kDa (mouse MAPK3) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).