Analysis Note

ControlHeLa cell lysate.

Application

Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryHistonesChromatin Biology

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.

Immunoprecipitation:
Recommended.

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)

Immunofluorescence:
Recommended.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone demethylase that specifically demethylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted ′Lys-4′ of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 ′Lys-4′ on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate ′Lys-9′ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Immunogen

GST fusion protein corresponding to residues 23-138 of human LSD1.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: CBL770

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.

Unpurified

Quality

Routinely evaluated by western blot.

Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.

Specificity

Recognizes LSD1, Mr 110 kDa.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

110 kDa