Analysis Note

ControlAcid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).

Application

Research Sub CategoryHistones

Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.

Research CategoryEpigenetics & Nuclear Function

Epitope Mapping (using Luminex^®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex™ microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

This antibody recognizes histone H4 independant of any post-translational modifications.

Immunogen

Epitope: a.a. 25-28

KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Luminex is a registered trademark of Luminex Corp

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.

Format: Purified

Quality

Routinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)

Specificity

Broad species cross-reactivity expected based on sequence homology.

Recognizes Histone H4, Mr 10 kDa.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

10 kDa