Analysis Note

ControlHuman IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.

Application

Immunocytochemistry: A previous lot was used by an independent laboratory in Phoenix cells transfected with an expression vector.

Immunohistochemistry: A previous lot of this antibody was used to detect IDO in human retina, primary eye tumor and metastatic liver tumor tissue sections (Chen, P.W., et al. Experimental Eye Research 85(2007) 617-625).

Anti-IDO (Indoleamine 2, 3-Dioxygenase) Antibody, clone 10.1 is an antibody against IDO (Indoleamine 2 for use in IC & WB.

General description

Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

Immunogen

GST fusion protein corresponding to residues 78-184 of human Indoleamine 2,3- Dioxygenase (IDO). Clone 10.1.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1056

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Quality

Routinely evaluated by Western Blot on untreated and IFNgamma treated (24 hrs) Hela lysates.

Western Blot Analysis:
1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.

Specificity

Recognizes IDO (Indoleamine 2,3- Dioxygenase), Mr 42 kDa.

Target description

42 kDa