Application

Research Sub CategoryCytoskeleton

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^® 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

Immunogen

Epitope: C-terminal 25 amino acids.

Purified chicken brain A-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Protein G Purified

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.

Specificity

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed.