Analysis Note

ControlA431 cell lysate.

Application

Immunofluorescence:
(Breitling, F., 1986)

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Immunogen

Epitope: a.a. 426-450

Purified human brain A-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1117

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol.

Protein G Purified

Format: Purified

Quality

Routinely evaluated by western blot on non-stimulated A431 lysate.

Western Blot Analysis:
0.1-1 µg/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue.

Specificity

Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

50-60 kDa