Application

Detect phospho-Akt (Thr308) using this Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25 validated for use in WB, IF, IC, ELISA.

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategorySignaling

Western Blot Analysis:
This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

Immunogen

KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-802

Physical form

Protein A purified

Protein A purified recombinant rabbit monoclonal supernatant in 0.1M Tris-Glycine (pH7.4) 150mM NaCl, and 0.05% NaN3 with 40% glycerol.

Format: Purified

Quality

This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

Specificity

Detects Akt only when phosphorylated on Thr308.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

60 kDa