Application

Detect phospho-SMC1 (Ser957) with Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 (Mouse Monoclonal Antibody), that has been shown to work in WB.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SMC1 is a ATPase and subunit of the multiprotein cohesin complex, a protein involved in chromosome segregation and in double-strand DNA break (DSB) repair. Serine 957 is phosphorylated by ATM in response to DSBs and is involved in the S-phase DNA damage checkpoint.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 951-962 (SQEEGS[pS]QGEDS) of human SMC1

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Purified

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Format: Purified

Quality

routinely evaluated by immunoblot on RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells

Specificity

phospho-SMC1 (Ser957)

Predicted to cross-react with xenopus and bovine based on sequence homology

Storage and Stability

2 years at -20°C

Target description

Mr 150kDa