Analysis Note

ControlK562 nuclear extract

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Detect Meis 1/2/3 with Anti-Meis 1/2/3 Antibody, clone 9.2.7 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Hox, Pbx, and Meis families of transcription factors form heteromeric complexes and bind DNA through specific homeobox domains. Meis1, 2, and 3 are members of the TALE (three amino acid loop extension) family of homeodomain containing proteins. Meis1 binds to Hox and Pbx proteins to form complexes with distinct DNA-binding specificities. Like Hox and Pbx proteins, the overexpression of Meis1 in BHX-2 myeloid leukemias implies a role for this protein in oncogenesis. Two Meis related proteins, Meis2 and Meis3 (also designated Mrg1 and Mrg2, respectively), possess largely similar sequence identity with Meis1 and express in normal tissues as well as myeloid leukemias. In the pancreas, Meis2 preferentially associates with Pbx1, and together they associate with the pancreas specific homeodomain factor, Pdx1, to repress Pdx1-induced transcriptional activation.

Immunogen

MBP-conjugated fusion protein corresponding to amino acids 60-390 of mouse Meis1

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABN1071

Physical form

Ascites

mouse ascites IgG1 containing 0.05% sodium azide before the addition of glycerol to 30%

Quality

routinely evaluated by immunoblot on nuclear extract from K562 cells

Specificity

Meis1, Meis2, Meis3 and splice variants

Storage and Stability

2 years at -20°C

Target description

Mr ~45-66kDa