Application

Western Blotting Analysis: A representative lot from this antibody detected SH3KBPI in NG108-15 cell lysate.

Research CategoryApoptosis & Cancer

Anti-SH3KBPI, clone 179.1.E1, Cat. No. 05-731-I, is a mouse monoclonal antibody that detects SH3 domain-containing kinase-binding protein 1 and has tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SH3 domain-containing kinase-binding protein 1 (UniProt: Q925Q9; also known as Regulator of ubiquitous kinase, Ruk, SH3-containing expressed in tumorigenic astrocytes, SH3KBPI,) is encoded by the Sh3kbp1 (also known as Ruk, Seta) gene (Gene ID: 84357) in rat. SH3KBPI is a peripheral membrane protein that serves as an adapter protein involved in regulating diverse signal transduction pathways. It localizes in endocytic vesicles containing clustered receptors. It is shown to be highly expressed in the spinal cord. It can self-associate and form homotetramers. It contains three SH3 domains (aa 1-58; 98-157; and 311-372). SH3KBPI is involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. It can attenuate phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. It is also reported to plays a role in the regulation of cell morphology and cytoskeletal organization. Seven different isoforms of SH3KBP1 have been described that are produced by alternative splicing.

Immunogen

His-tagged full-length recombinant rat SH3 domain-containing kinase-binding protein 1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SH3KBPI in THP-1 cell lysate.

Specificity

Clone 179.1.E1 is a mouse monoclonal antibody that specifically detects SH3 domain-containing kinase-binding protein 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85 kDa observed; 78.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).