Application

This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor^® 555 is validated for use in Immunocytochemistry, Immunohistochemistry for the detection of Active-β-Catenin.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in NIH/3T3 cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Active-β-Catenin in human colon and human colorectal adenocarcinoma tissue.
The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.

Immunogen

Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.

Epitope: The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.

Specificity

The antibody eptiope is 100% conserved in zebrafish.

Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85 kDa observed