Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor^® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.

Immunogen

Linear peptide corresponding to phospho Histone H2A.X (Ser139).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.

Protein G Purified

Quality

Evaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.