Application

This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor^® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).

General description

Histone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H2A.X (Ser139) in Staurosporin treated HeLa cells, and not in untreated HeLa cells.

Target description

17 kDa calculated