Analysis Note

ControlPancreas. RIPA cell lysates

Application

Anti-CFTR Antibody, clone MM13-4 detects level of CFTR & has been published & validated for use in IC, IP & WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Immunoprecipitation:
This antibody has been shown to immunoprecipitate CFTR from a T84 cell lysate by an independent laboratory.


Immunocytochemistry:
This antibody has been reported to immunostain CFTR in T84 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CFTR (Cystic Fibrosis Transmembrane conductance Regulator) is a 170 kDa chloride (Cl-) channel that regulates salt and water transport across epithelial tissues, where it modulates production of mucus, sweat, saliva, tears, and digestive enzymes. Cystic fibrosis patients have mutations in this protein, resulting in defective in ion transport. Symptoms include reduced transport of sodium chloride (saltier sweat) and digestive and respiratory disorders caused by thicker mucus. CFTR mutations are also associated with congenital bilateral absence of the vas deferens in males.

Immunogen

Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, cow and frog, 10/12 identity in mouse and 9/12 identity in rat. Clone MM13-4.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1k in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.

Protein A purified

Format: Purified

Quality

Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or CFTR-transfected BHK.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR in human T84 colon carcinoma epithelial RIPA cell lysates. A previous lot detected CFTR in CFTR-transfected BHK.

Note: CFTR can run as a diffuse protein on SDS-PAGE.

Specificity

This antibody recognizes CFTR, Mr 170kDa. Two additional proteins were detected, Mr 52 and 38 kDa, in CFTR-transfected BHK cell lysates.

Other species cross-reactivity not tested.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

170 kDa