Analysis Note

ControlPositive Control Included: 3T3 Cell Lysate (12-305). Other controls: A31 cell lysate, PC-12, Hep-G2, SW 620 and Jurkat RIPA cell lysates

Application

Research CategorySignaling

Anti-FAK Antibody, clone 4.47 is an antibody against FAK for use in IC, IH, IP & WB.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.

Immunogen

GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-591

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%

Quality

Routinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates

Specificity

Recognizes p125FAK; does not cross react with Pyk-2.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

125 kDa