Analysis Note

ControlJurkat cell lysate and human cervical carcinoma.
Included Positive Antigen Control: Catalog # 12-303, Jurkat cell extract. Use 20 µg per lane for minigels.

Application

Anti-IKKβ Antibody, clone 10AG2 detects level of IKKβ & has been published & validated for use in WB.

General description

IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1.

Immunogen

His-tagged, full length human IKKβ/IKK2. Clone: 10AG2.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS and 0.05% sodium azide. Frozen solution.

Specificity

This antibody recognizes IKKβ/IKK2, Mr 87 kDa.

Other species not tested.

Target description

87 kDa