Analysis Note

ControlHeLa cell extract

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Anti-Histone H3 Antibody, clone 6.6.2 is a Mouse Monoclonal Antibody for detection of Histone H3 also known as H3 histone family 3A & has been validated in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This monoclonal antibody was generated using an unmodified peptide corresponding to the first 20 amino acids of histone H3 and is specific for an unmodified epitope in that region.

Immunogen

Epitope: a.a. 1-20

KLH conjugated peptide corresponding to amino acids 1-20 [ARTKQTARKSTGGKAPRKQLC] of human histone H3. Clone 6.6.2.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.

Protein G Chromatography

Format: Purified

Quality

Routinely evaluated by western blot on acid extracted lysates from HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Histone H3 in acid extracted lysates from HeLa cells.

Specificity

This antibody recognizes and is specific for histone H3, Mr 17kDa.

This peptide sequence is identical in a wide range of animal and plant species.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Target description

17 kDa