Analysis Note

ControlIncluded Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 µl of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels

Application

Research CategoryProtein Trafficking

This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Immunocytochemistry:
5-10 µg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.

Immunoprecipitation:
2 µg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK′s, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl′s ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.

Immunogen

Epitope: a.a. 878-906

GST fusion protein corresponding to residues 878-906 of human Cbl

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4

Protein G Chromatography

Format: Purified

Quality

routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11

Specificity

Antibody recognizes amino acids 878-906 on human Cbl

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target description

120kDa