Application

Anti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB.

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.

Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.

Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.

General description

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Immunogen

Epitope: The epitope maps to a region (a.a. 834-864) common to all NR1 isoforms

His-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on rat brain microsomal protein preparation.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected NR1 from 20 µg of rat brain microsomal protein preparation (Catalog # 12-144).

Specificity

This antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.

Storage and Stability

Stable for 1 year at 2° to 8°C from date of receipt.

Target description

130 kDa observed