Application

Research CategorySignaling

Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor^® 488 Conjugate is an antibody against PP2A for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PP2A in NIH/3T3 cells and HUVECs.
The unconjugated antibody (Cat. No. 05-421) is shown to be suitable also for immunocytochemistry, immunoprecipitation, and Western blotting applications.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (EC 3.1.3.16; UniProt P67775; PP2A-alpha, PP2Ac, PP2Calpha, Replication protein C, RP-C) is encoded by the PPP2CA (also known as PP2CA) gene (Gene ID 5515) in human. Protein phosphatase 2 (PP2 or PP2A) is a ubiquitously expressed heterotrimeric serine/threonine protein phosphatase with broad substrate specificity, such as Raf, MEK, and AKT. PP2A is composed of a structural A and a catalytic C subunit as the dimeric core enzyme, and a regulatory B subunit. There exist two different A subunits (PPP2R1A & PPP2R1B), two different C subunits (PPP2CA & PPP2CB), and 12 different B subunits (PPP2R2B-D, PPP2R3A-C, PPP2R4, PPP2R5A-E). The enzymatic activity of a catalytic subunit is altered upon binding an A subunit even in the absence of a B subunit. The B subunits are believed to play key roles in controlling the localization and specific activity of the A/C holoenzymes.

Immunogen

A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A).

Epitope: Amino acids 295-309.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified mouse monoclonal IgG2bκ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected PP2A in A431 cells.

Specificity

Expected to recognize both spliced isoforms of human PP2A catalytic subunit reported by UniProt (P67775).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~36 kDa observed. 35.59 kDa (isoform 1) and 29.74 kDa (isoform 2) calculated.