Analysis Note

ControlPositive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Application

Research Sub CategoryEpitope Tags

Anti-Myc Tag Antibody, clone 9E10 is an antibody against Myc Tag for use in IH(P), IC, IP & WB.

Research CategoryEpitope Tags & General Use

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties. r>The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Immunogen

Synthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNS).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Protein G Purified

Format: Purified

Quality

routinely evaluated by immunoblot on a Myc-tagged recombinant protein

Specificity

Recognizes Myc and recombinant proteins containing the Myc epitope tag.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

Predicted MW for human Myc: 49 kDa