Analysis Note

ControlWestern Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells

Application

Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.

Research CategorySignaling

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.

Immunogen

Full length GST fusion protein corresponding to Human CRKL

Epitope: Full-length

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Purified

Format: Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Quality

Routinely evaluated by Western Blot on K562 cell lysate.

Western Blot Analysis: Antibody detected CRKL in 15 µg of K562 cell lysate.

Specificity

Other species have not been tested.

the C-terminal SH3 of Human CRKL

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Target description

39kDa