Application

Research CategorySignaling

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.

Research Sub CategoryDevelopmental Signaling

This Anti-Rac1 Antibody, clone 23A8, Alexa Fluor^® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Rac1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions, including cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family consists of 22 members, which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamily (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.

Immunogen

Recombinant protein containing the full length human Rac.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in A431 cells.

Specificity

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~21 kDa observed