Analysis Note

ControlPositive Control Included: Jurkat cell lysate (12-303)

Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

For testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf

This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Immunogen

GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Format: Purified

Quality

routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells

Specificity

Recognizes human ZAP-70, does not recognize murine ZAP-70.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

70 kDa