Analysis Note

ControlJurkat cell lysate.
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.

Application

Research CategoryProtein Trafficking

Detect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB.

Research Sub CategoryRNA Metabolism & Binding ProteinsRNA Binding Protein (RBP)

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.

Immunogen

Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Chromatography

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.

Quality

Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.

Specificity

This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

53 kDa