Analysis Note

ControlImmunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine

Application

Anti-Nitrotyrosine Antibody, clone 1A6 detects level of Nitrotyrosine & has been published & validated for use in IC, IH, IP & WB.

General description

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.

Format: Purified

Quality

routinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins

Target description

Predicted MW for nitrated-BSA: 66 kDa