Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetPotent vasoconstrictive action

General description

A 21 amino acid polypeptide with potent vasoconstrictive action. Produces convulsions, arterial hypertension, and metabolic activation of periventricular white and gray matter. Its effects are mediated via L-type Ca²⁺ channels. Reported to activate phospholipase C in fibroblasts expressing ET_A receptors.

A 21-amino acid polypeptide with potent vasoconstrictive action. Produces convulsions, arterial hypertension, and metabolic activation of periventricular white and gray matter. Its effects are mediated via L-type Ca²⁺ channels. Reported to activate phospholipase C in fibroblasts expressing ET_A receptors.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cesari, M., et al. 1996. Am. Heart J.132, 1236.Gresser, O., et al 1996. Biochem. Biophys. Res. Commun.224, 169.Murata, S., et al. 1995. J. Pharmacol. Exp. Ther. 274, 1524.Hexum, T.D., et al. 1990. Biochem. Biophys. Res. Commun. 167, 294.Itoh, Y., et al. 1988. FEBS Lett. 231, 440.Yanagisawa, M., et al. 1988. Nature 332, 411.

Packaging

Yes

Physical form

Supplied as an acetate salt.

Preparation Note

Dissolve peptide initially at 8 mg/ml in 80% cold acetic acid, then dilute with distilled water to achieve 5% acetic acid and 0.5 mg/ml peptide.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.

Sequence

H-Cys¹-Ser-Cys³-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys¹¹-Val-Tyr-Phe-Cys¹⁵-His-Leu-Asp-Ile-Ile-Trp-OH (disulfide bonds: 1 → 15; 3 → 11)

Warning

Toxicity: Standard Handling (A)