Application

Anti-Src Antibody, clone GD11, Alexa Fluor^® 488 conjugate is an antibody against Src for use in Immunocytochemistry.

Research Sub CategoryOncoproteins

Research CategoryCell Structure

Immunocytochemistry Analysis: An 1:100 dilution of this antibody from a representative lot detected Src in A431, HUVECs, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine-protein kinase transforming protein Src (EC 2.7.10.2; UniProt P00526; also known as p60-Src, pp60v-src, v-Src) is encoded by the V-SRC gene (Gene ID 1491925) of Rous sarcoma virus strain Prague C (RSV-PrC).
Rous sarcoma virus (RSV) V-SRC gene is the first retroviral oncogene discovered. V-SRC encodes the v-Src tyrosine kinase that transforms infected host cells into uncontrolled mitosis, providing abundant cells for fresh infection. The non-oncogenic chicken c-Src (cellular Src) gene structurally related to v-Src was discovered later in 1979 by J. Michael Bishop and Harold E. Varmus. It is believed that RSV originally took up the chicken proto-oncogene SRC (Gene ID 396442; UniProt P00523) and incorporated into its genome in the mutated concogene form. c-Src contains six functional regions, Src homology 4 (SH4) domain, unique region, SH3 domain, SH2 domain, catalytic domain, and short regulatory tail. In its inactive state, Src is phosphorylated at Tyr527. The pTyr527 interacts with the SH2 domain which helps the SH3 domain interact with the linker domain and consequently keeps the inactive unit tightly bound. Dephosphorylation of pTyr527 destabilizes the inactive conformation, opening up the SH3, SH2 and the kinase domains, leading to Tyr416 autophosphorylation and c-Src activation.

Immunogen

Epitope: Amino acids 82-169.

Recombinant PrA-RSV p60src.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Protein G Purified

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: An 1:100 dilution of this antibody detected Src in HeLa cells.

Specificity

Clone GD11 reacted with v-Src of Rous sarcoma virus (RSV) strains Prague subgroup A (PrA-RSV), Schmidt-Ruppin subgroup D (SRD-RSV), and Bratislava 77 subgroup C (B77 C-RSV). Clone GD11 detected enzymatically active c-Src from a variety of chicken, rodent, and human cells (Parsons, S.J., et al. (1984). J. Virol. 51(2):272-82).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed.