Analysis Note

ControlIncluded Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. Use 20 µg per lane for minigels.

Application

Research CategorySignaling

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay:
Use 10 µg/mL to detect calmodulin in the range of 200-300 ng.

Detect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

Immunogen

Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.

Epitope: a.a. 128-148

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1158

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Chromatography

Format: Purified

Purified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

Quality

Evaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Western Blot Analysis: 0.1-1 µg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Specificity

Also reacts to wheat germ. Wide cross-reactivity expected.

Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.

Storage and Stability

Stable for 1 year at from date of receipt.

Target description

17 kDa