Analysis Note

ControlMCF-7 cell extracts

Application

Research CategorySignaling

Immunoprecipitation:
5 µg of a previous lot immunoprecipitated 100 ng of IGF-I.

Immunohistochemistry:
10 µg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections.
(Lui, 1994).

Neutralization:
10-20 µg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments).

Research Sub CategoryInsulin/Energy Signaling

Anti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges.
IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Immunogen

Human insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse IgG1κ lyophilized powder, lyophilized from 105 µL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA.

Quality

Evaluated on IGF-I (Catalog # 01-208) under non-reducing conditions.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions.

Specificity

IGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997).

Possibly chicken. Occasional difficulty neutralizing rat IGF-I due to binding protein interference.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

17 kDa, migrates to ~7 kDa