Analysis Note

ControlHeLa cell lysate

Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Immunohistochemistry Analysis:
A representative lot was used by an independent laboratory in IH.

Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in IP.

Anti-PRL-2 Antibody, clone 42 detects level of PRL-2 & has been published & validated for use in WB, IH & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PRL-2 is one of three members (PRL-1, PRL-2, and PRL-3) that is part of a unique protein-tyrosine phosphatase ((PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP is known to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferres a transformed phenotype and can result in a tumor growth in nude mice, suggesting a role in tumorigenesis. Alternatively spliced transcript variants that encode two distinct isoforms have been described

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to mouse PRL-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected PRL-2 on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes PRL-2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 19 kDa