Analysis Note

ControlHeLa cell lysates.

Application

Anti-Gemin2 Antibody, clone 2E17 is a Mouse Monoclonal Antibody for detection of Gemin2 also known as SMN interacting protein 1-delta & has been validated in WB, ICC, ELISA & IP.

Research Sub CategoryRNA Metabolism & Binding ProteinsNeurodegenerative Diseases

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry:
Cited in Battle, D.J., et. al (2007). J. Biol. Chem. 282: 27953 - 27959 and Zhang, Z., et. al (2008). Cell, 133:85-600. using a representative lot.
ELISA:
Cited in Zhang, Z., et. al (2008). Cell, 133:85-600.
Immunoprecipitation:
Cited in Battle, D.J., et. al(2007). J. Biol. Chem. 282: 27953 - 27959 using a representative lot.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Gemin-2 (also known as SIP1 or SMN interacting protein1) is a part of the SMN complex. The SMN complex plays a role in RNA metabolism and in splicing regulation. As part of the SMN complex Gemin-2 interacts directly with Gemin-5 in a heteromeric complex that includes Gemin-3, Gemin-4, Gemin-6. This macromolecular complex (SMN complex) mediates assembly of spliceosomal snRNA complexes containing U1 and U5.

Immunogen

His-tagged recombinant protein corresponding to human Gemin-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide

Quality

Evaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Gemin-2 in HeLa cell lysates.

Specificity

Antibody recognizes Gemin-2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

32 kDa Calculated