Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryRNA Binding Protein (RBP)

Research CategoryEpigenetics & Nuclear Function

Anti-Y14 Antibody, clone 4C4 is a Mouse Monoclonal Antibody for detection of Y14 also known as Binder of OVCA1-1, RNA binding motif protein 8A, Ribonucleoprotein RBM8A & has been validated in WB, ICC & IP.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Y14 in HeLa and NIH/3T3 cells.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in immunoprecipitation. Kashima, I., et al. (2006). Genes & Dev. 20:355-367.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Y14 (RNA binding motif protein 8A or RBM8A), an RNA-binding protein, contains a conserved RNA-binding motif. Y14 is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

Immunogen

Epitope: Unknown

Purified human Y14.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Y14 on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes Y14.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

24 kDa