Analysis Note

ControlJurkat cell lysate

Application

Research CategorySignaling

Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue.

ELISA: A previous lot of this antibody was used in ELISA.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunocytochemistry: A previous lot of this antibody was used in IC.

Flow Cytometry: A previous lot of this antibody was used in FC.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryGrowth Factors & Receptors

Anti-Endoglin Antibody, clone P3D1 is an antibody against Endoglin for use in WB, IH, IC, IP, FC, ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Endoglin is a homodimeric integral membrane glycoprotein comprised of 95 kDa subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-β) family ligands to signaling receptors. Endoglin binds TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. It is also expressed by U937 cells and activated macrophages. Endoglin expression is up regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

Immunogen

Human resting umbilical vein endothelial cells.

Linkage

Replaces: MAB2152

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Format: Purified

Purified mouse monoclonal IgG2a in 0.02M phosphate buffer, 0.25M sodium chloride, 0.1% sodium azide, pH 7.6

Quality

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ENDOGLIN on 10 µg of Jurkat lysates.

Specificity

Reacts with human endoglin.

Storage and Stability

Stable at 2-8°C for up to 1 year from date of receipt.

Target description

~71 kDa