Analysis Note

ControlMouse colon tissue

Application

Anti-CD45 Antibody, clone IBL-5/25 is an antibody against CD45 for use in FC, IH.

Flow Cytometry Analysis: A previous lot detected CD45, clone IBL-5/25 in mouse peripheral blood lymphocytes

General description

CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Immunogen

Mouse lymph node cells.

Linkage

Replaces: CBL1326

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG in phosphate buffered saline containing 10 mM sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry in mouse colon tissue.
Immunohistochemistry Analysis: 1:100 dilution of this antibody detected CD45 in mouse colon tissue.

Specificity

This antibody reacts with all isoforms of mouse CD45 which is expressed on all haematopoietic cells except erythrocytes.
FUSION PARTNER: SP2/0 myeloma cell line

Reactivity with other species has not been determined.

Storage and Stability

Store at 2-8°C for 12 months from date of receipt.

Target description

Approx. 147 kDa