Analysis Note

ControlRAW264 Cell Lysate

Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Immunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining.

Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.

Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.

Optimal Staining of CD45RA Monoclonal Antibody: Tonsil

Anti-CD45RA Antibody, Clone MEM 56 detects level of CD45RA & has been published & validated for use in FC, IF, IH(P), IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Linkage

Replaces: 04-1102

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Purified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
We recommend that each laboratory determine an optimum working titre for use in its particular application.

Format: Purified

Quality

Routinely evaluated by Western Blot on RAW 264 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 µg of RAW 264

Specificity

This antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems.
Antigen distribution:
B-cells (lg+) >95%
T-cells (E+) 70 + 10%
Peripheral blood lymphocytes 68 + 8%
T-cells (CD4+) 50 + 10%
Monocytes 50 + 20%
Thymocytes ﹤5%
Granulocytes ﹤1%

Storage and Stability

For use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~220 kDa