Analysis Note

ControlHEK293 cell lysate

Application

Research Sub CategoryTranscription Factors

Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used in immunocytochemistry on NIH/3T3 and A431 cells.

Use Anti-RING1 Antibody, clone 4D6 (Mouse Monoclonal Antibody) validated in WB, ICC to detect RING1 also known as Polycomb complex protein RING1.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RING1 belongs to the RING finger family, members of which are characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain.

Immunogen

GST-tagged recombinant protein corresponding to RING1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.

Quality

Evaluated by western blot on HEK293 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected RING1 in HEK293 cell lysate.

Specificity

This antibody detects RING1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~42 kDa