Analysis Note

ControlHepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate

Application

Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Immunogen

Epitope: C-terminus

Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Linkage

Replaces: MABE15

Physical form

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Unpurified

Quality

Evaluated by western blot in HepG2 cell lysate.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.

Specificity

Antibody recognizes Histone H2B near the C-terminus.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~15 kDa