Analysis Note

ControlHeLa Acid extract lysate.

Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

Use Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Immunogen

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.

Epitope: N-terminus (unmodified)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Mouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.

Peptide affinity chromatography

Quality

Routinely evaluated by Western Blot on HeLa cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 µg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.

Specificity

Broad species cross-reactivity is expected, based on sequence homology.

This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Approx. 17 kDa