Analysis Note

ControlHeLa Acid extract lysate

Application

Research Sub CategoryHistones

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex^® assay.

Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304, Trial Size is a mouse monoclonal antibody for detection of Trimethyl Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in ELISA, ICC, IP, WB, Mplex, DB.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5′ end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.

Immunogen

Epitope: Trimethylated Lys4

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.

Legal Information

Luminex is a registered trademark of Luminex Corp

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.

Quality

Western Blot Analysis:
0.5 - 2 µg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 µg of HeLa acid extract.

Specificity

Broad species cross-reactivity is expected, based on sequence homology.

This antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

approx. 17 kDa