Analysis Note

ControlHeLa cell lysate

Application

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.

Use Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates ′Lys-9′ and ′Lys-27′ residues of histone H3.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human SUZ12.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells.

Specificity

This antibody recognizes SUZ12.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 95 kDa