Analysis Note

ControlLys63 polyubiquitin chains.

Application

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.

Research CategorySignaling

Western Blot Analysis:
A 2 µg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications

Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.

Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer′s patient.

ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Immunogen

Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).

Epitope: Lys63 ubiquitination site

Physical form

Purified immunoglobulin by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.

Format: Purified

Quality

Western Blot Analysis:
A 2 µg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.

Specificity

Based on sequence homology, broad species cross-reactivity is expected.

Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target description

varies